GETTING MY HPLC USP TO WORK

The focus of polynuclear aromatic hydrocarbons (PAH) in soil are determined by initially extracting the PAHs with methylene chloride. The extract is diluted, if necessary, as well as the PAHs separated by HPLC employing a UV/Vis or fluorescence detector. Calibration is attained making use of a number of external standards. In a standard analysis a

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